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RSV/hGATA3
(Plasmid #83814)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83814 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RSV-CAT
  • Backbone manufacturer
    Gorman, et al. 1982
  • Modifications to backbone
    CAT was removed
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GATA3
  • Species
    H. sapiens (human)
  • Entrez Gene
    GATA3 (a.k.a. HDR, HDRS)
  • Promoter RSV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pREP-F (GCTCGATACAATAAACGCC)
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RSV/hGATA3 was a gift from Douglas Engel (Addgene plasmid # 83814 ; http://n2t.net/addgene:83814 ; RRID:Addgene_83814)
  • For your References section:

    Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD. Mol Cell Biol. 1991 May . 11(5):2778-84. PubMed 2017177