pcDNA3.1 HA-YY1
(Plasmid #104395)

• Purpose: HA-YY1
• Depositing Lab: Richard Young
• Publication: Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5800
• Total vector size (bp): 6700
• Modifications to backbone: Added HA tag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YY1
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• GenBank ID: NM_003403
• Entrez Gene: YY1 (a.k.a. DELTA, GADEVS, INO80S, NF-E1, UCRBP, YIN-YANG-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: T7_prom
• 3′ sequencing primer: BGH_rev

Resource Information

• Supplemental Documents:
  • pcDNA3-HA-YY1.gb
• A portion of this plasmid was derived from a plasmid made by: Yang Shi
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry