p209 pCMV-cre-K
(Plasmid #8394)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBS185
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Modified 5' Cre
  • Mutation
    Modified 5' region of Cre

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BssHII (unknown if destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Derived from plasmid p205 pCMV-cre-del. Shortening of 5' untranslated region of Cre from 484 to 19 bp, an optimized Kozak translational start site, introduction of an EcoR1 site at nucleotide 509 and a PstI site at nucleotide 718, amino acid changes (S2A, H8PEF).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p209 pCMV-cre-K was a gift from Jeffrey Green (Addgene plasmid # 8394)
  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679