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Plasmid 8394: p209 pCMV-cre-K
  • Modified 5' Cre

  • Modified 5' region of Cre

  • pBS185
    (Search Vector Database)

  • Mammalian Expression, Cre/Lox

  • 6100

  • XhoI

  • Unknown

  • BssHII

  • Unknown

  • na List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • View map

  • Jeffrey Green

  • UBMTA

Comments: 

Derived from plasmid p205 pCMV-cre-del. Shortening of 5' untranslated region of Cre from 484 to 19 bp, an optimized Kozak translational start site, introduction of an EcoR1 site at nucleotide 509 and a PstI site at nucleotide 718, amino acid changes (S2A, H8PEF).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8394" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only