Addgene - p209 pCMV-cre-K Plasmid Data

Plasmid 8394: p209 pCMV-cre-K

Gene/insert name:
Modified 5' Cre
Mutation:
Modified 5' region of Cre
Vector backbone:
pBS185
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
6100
Cloning site 5':
XhoI
Site destroyed during cloning:
Unknown
Cloning site 3':
BssHII
Site destroyed during cloning:
Unknown
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA

Comments:

Derived from plasmid p205 pCMV-cre-del. Shortening of 5' untranslated region of Cre from 484 to 19 bp, an optimized Kozak translational start site, introduction of an EcoR1 site at nucleotide 509 and a PstI site at nucleotide 718, amino acid changes (S2A, H8PEF).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8394" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

8395: p210 pCMV-CREM

8832: pRK1035

8839: pKM999

11086: pDEST-periHisMBP

11085: pDEST-HisMBP