Addgene - p210 pCMV-CREM Plasmid Data

Plasmid 8395: p210 pCMV-CREM

Gene/insert name:
CREM
Alt name:
modified Cre
Mutation:
modified Cre
Vector backbone:
pBS185
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
6100
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
BssHII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA

Comments:

Derived from p209 pCMV-cre-K. Contains altered human beta-globin intron from plasmid pCI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8395" in your Materials and Methods section.