|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8395||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Alt namemodified Cre
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site BssHII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Derived from p209 pCMV-cre-K. Contains altered human beta-globin intron from plasmid pCI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p210 pCMV-CREM was a gift from Jeffrey Green (Addgene plasmid # 8395 ; http://n2t.net/addgene:8395 ; RRID:Addgene_8395)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679