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p210 pCMV-CREM
(Plasmid #8395)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8395 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS185
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CREM
  • Alt name
    modified Cre
  • Mutation
    modified Cre

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BssHII (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Derived from p209 pCMV-cre-K. Contains altered human beta-globin intron from plasmid pCI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p210 pCMV-CREM was a gift from Jeffrey Green (Addgene plasmid # 8395 ; http://n2t.net/addgene:8395 ; RRID:Addgene_8395)
  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679