Addgene - p212 pCMV-EGFP/RFP Plasmid Data

Plasmid 8389: p212 pCMV-EGFP/RFP

Gene/insert name:
floxed EGFP
Alt name:
loxI EGFP loxII
Insert size:
800
Vector backbone:
pDsRed1-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
4700
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

EGFP-RFP gene switch plasmid. Floxed EGFP gene switch cassette (KpnI fragment
from plasmid "p144").

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8389" in your Materials and Methods section.