Addgene - p133 pSV-STOP-luc Plasmid Data

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Plasmid 8390: p133 pSV-STOP-luc

Gene/insert name:
floxed STOP
Alt name:
loxI STOP loxII
Vector backbone:
pGL3 Control
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase, Cre/Lox
Backbone size w/o insert:
5200
Cloning site 5':
AvrII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
RVprimer3 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

See author's map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8390" in your Materials and Methods section.