Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8386||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6800
Vector typeMammalian Expression, Cre/Lox ; Beta-gal
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namefloxed EGFP
Alt nameloxI EGFP loxII
Insert Size (bp)1100
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Terms and Licenses
Floxed EGFP gene switch cassette (KpnI fragment
from plasmid "p142") driven by SV40 promoter.
Note that a there is a second Kpn1 site within beta gal resulting in KpnI digests that yield bands of 6kb, 1.1kb, and approximately .6kb.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p144 pSV-EGFP/beta-gal was a gift from Jeffrey Green (Addgene plasmid # 8386 ; http://n2t.net/addgene:8386 ; RRID:Addgene_8386)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679