Addgene - p155 pCMV-EGFP/beta-gal Plasmid Data

Plasmid Cart

Login to add to cart

Recently Visited

8387: p155 pCMV-EG…
8385: p142
8328: pgex-cIAP1
34610: pLJM60
32447: CMV-G-GECO1.…

Popular Plasmids

Special Collections

Analyze Sequence

Vector Database

Plasmid 8387: p155 pCMV-EGFP/beta-gal

Gene/insert name:
floxed EGFP
Alt name:
loxI EGFP loxII
Insert size:
800
Vector backbone:
pCMV-beta-gal
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Cre/Lox
Vector type:
Beta-gal
Backbone size w/o insert:
7164
Cloning site 5':
SmaI
Site destroyed during cloning:
Yes
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA

Comments:

StuI/SalI fragment from pSV-EGFP/beta-gal. Floxed EGFP gene switch cassette driven by CMV.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8387" in your Materials and Methods section.