Purpose(Empty Backbone) Modified from pCFD4, with the vermillion marker and the attB site removed
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83954||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSimon Bullock
- Backbone size (bp) 4575
Modifications to backboneremoved the vermillion marker and the attB site
- Promoter dU6-1; dU6-3
Growth in Bacteria
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ATTCCTATCCGGCGGTAGTC
- 3′ sequencing primer TGTACGTCAACGGAAAACCA (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFD4d was a gift from Phillip Zamore (Addgene plasmid # 83954 ; http://n2t.net/addgene:83954 ; RRID:Addgene_83954)
For your References section:Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion. Ge DT, Tipping C, Brodsky MH, Zamore PD. G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 10.1534/g3.116.032557. 10.1534/g3.116.032557 PubMed 27543296