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pUC57-white[coffee]
(Plasmid #84006)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84006 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GenScript, Inc.
  • Backbone size w/o insert (bp) 2517
  • Total vector size (bp) 4597
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A 2,080 bp fragment of the white[coffee] allele
  • Alt name
    CG2759
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2080
  • Mutation
    A GC-to-AA mutation that creates a G589E missense mutation; silent mutations to confer resistance to white sgRNAs-1, -2, -3 and -4
  • Entrez Gene
    w (a.k.a. Dmel_CG2759, BACN33B1.1, CG2759, DMWHITE, Dmel\CG2759, EG:BACN33B1.1, W, c23, e(g), m(g), mw, w(AT)[[13]])

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer M13_F
  • 3′ sequencing primer M13_R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-white[coffee] was a gift from Phillip Zamore (Addgene plasmid # 84006 ; http://n2t.net/addgene:84006 ; RRID:Addgene_84006)
  • For your References section:

    Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion. Ge DT, Tipping C, Brodsky MH, Zamore PD. G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 10.1534/g3.116.032557. 10.1534/g3.116.032557 PubMed 27543296