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pHD066
(Plasmid #84032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84032 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cre
  • Promoter Elastase
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Venus
  • Promoter Crystalin

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

Designed in a pBluescript series vector containing I-SceI meganuclease sites. The I-SceI meganuclease method is a common method for establishing transgenic zebrafish lines.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHD066 was a gift from Daniel Hesselson & Didier Stainier (Addgene plasmid # 84032 ; http://n2t.net/addgene:84032 ; RRID:Addgene_84032)
  • For your References section:

    Suppression of Ptf1a activity induces acinar-to-endocrine conversion. Hesselson D, Anderson RM, Stainier DY. Curr Biol. 2011 Apr 26;21(8):712-7. doi: 10.1016/j.cub.2011.03.041. Epub 2011 Apr 14. 10.1016/j.cub.2011.03.041 PubMed 21497092