pHD066
(Plasmid #84032)

• Purpose: Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafish
• Depositing Labs: Daniel Hesselson, Didier Stainier
• Publication: Hesselson et al Curr Biol. 2011 Apr 26;21(8):712-7. doi: 10.1016/j.cub.2011.03.041. Epub 2011 Apr 14.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	84032	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: unknown
  (Search Vector Database)
• Vector type: Cre/Lox ; Zebrafish transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cre
• Promoter: Elastase
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: mCherry-F (ccccgtaatgcagaagaaga)

Gene/Insert 2

• Gene/Insert name: Venus
• Promoter: Crystalin

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pHD066-map
• Terms and Licenses:
  • UBMTA
  • Clontech Limited Use Label License

Depositor Comments

Designed in a pBluescript series vector containing I-SceI meganuclease sites. The I-SceI meganuclease method is a common method for establishing transgenic zebrafish lines.