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Plasmid 8404: p236 pPr-CREM
  • probasin driven CREM

  • modified Cre

  • modified Cre

  • pbeta-gal-Basic
    (Search Vector Database)

  • Clontech

  • Mammalian Expression, Cre/Lox

  • 7500

  • XhoI

  • No

  • EcoRI

  • No

  • na List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • View map

  • Jeffrey Green

  • UBMTA

Comments: 

428 bp probasin promoter fragment from prPb-tva950 (Pam Schwartzenberg, NCI), CREM from p210 pCMV-CREM, coding sequence for beta-gal removed.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8404" in your Materials and Methods section.