Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8405||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameprobasin driven CREM with insulators
Alt namemodified Cre
- Cloning method Restriction Enzyme
- 5′ cloning site DraIII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
428 bp probasin promoter fragment from prPb-tva950 (Pam Schwartzenberg, NCI), CREM from p210 pCMV-CREM, pair of chicken globin insulators from plasmid pJC13.1 (G. Felsenfeld, NCI), coding sequence for beta-gal removed.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p240 pPr-CREM/Ins was a gift from Jeffrey Green (Addgene plasmid # 8405 ; http://n2t.net/addgene:8405 ; RRID:Addgene_8405)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
Map uploaded by the depositor.