Addgene - p255 pPr-CREM/Ins/CMV-STOP-luc Plasmid Data

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Plasmid 8408: p255 pPr-CREM/Ins/CMV-STOP-luc

Gene/insert name:
CREM/insulators/lox/luciferase
Alt name:
modified Cre/lox
Mutation:
modified Cre
Vector backbone:
pbeta-gal-Basic
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert:
7500
Cloning site 5':
EcoRICI
Site destroyed during cloning:
Yes
Cloning site 3':
DraIII
Site destroyed during cloning:
Yes
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Chicken globin insulators between Pr-CREM and CMV-STOP-luc cassettes. Pr-CREM from p240 pPr-CREM/Ins isolated with DraIII and Eco47III and ligated into EcoRICI/DraIII fragment of pCMVe-betaAc-STOP-luc (p231).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8408" in your Materials and Methods section.