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p252 pPr-CREM/CMV-STOP-luc
(Plasmid #8406)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8406 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pbeta-gal-Basic
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CREM/lox/luciferase
  • Alt name
    modified Cre/lox
  • Mutation
    modified Cre

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site DraIII (not destroyed)
  • 3′ cloning site EcoR47III (destroyed during cloning)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

DraIII and EcoICRI fragment containing the CMV-STOP-luc cassette from p231 pCMVe-betaAc-STOP luc ligated to the DraIII and Eco47III fragment from p236 pPr-CREM containing probasin-modified cre.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p252 pPr-CREM/CMV-STOP-luc was a gift from Jeffrey Green (Addgene plasmid # 8406 ; http://n2t.net/addgene:8406 ; RRID:Addgene_8406)
  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679