Addgene - Mouse c-myc (exons II and III) Plasmid Data

Plasmid 8448: Mouse c-myc (exons II and III)

Gene/insert name:
Mouse c-myc (exons II and III)
Alt name:
myc
Alt name:
c-myc
Insert size:
1400
Species:
M. musculus (mouse)
Entrez Gene:
Myc (AU016757, Myc2, Niard, Nird, bHLHe39)
Mutation:
Exons II and III only
Vector backbone:
pSP64
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3000
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
M13 Reverse List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Recloned from Kenneth Marcu's M54 cDNA described in Nature 1984 Aug 2-8;310(5976):423-5.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Frederic Mushinski
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Addition of constitutive c-myc expression to Abelson murine leukemia virus changes the phenotype of the cells transformed by the virus from pre-B-cell lymphomas to plasmacytomas. Weissinger et al (Mol Cell Biol 1993 Apr;13(4):2578-85. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8448" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

12260: psPAX2

12259: pMD2.G

9050: pBABE puro cyclinD1 HA

8446: Mouse c-myc (exon I)