Purposemammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85082||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer CMV forward primer (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-BLZinCh-1 was a gift from Maarten Merkx (Addgene plasmid # 85082 ; http://n2t.net/addgene:85082 ; RRID:Addgene_85082)
For your References section:Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc. Aper SJ, Dierickx P, Merkx M. ACS Chem Biol. 2016 Oct 21;11(10):2854-2864. Epub 2016 Sep 1. 10.1021/acschembio.6b00453 PubMed 27547982