Addgene - HS-H4-GFP Plasmid Data

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8699: HS-H4-GFP
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Plasmid 8699: HS-H4-GFP

Gene/insert name:
H4
Insert size:
320
Species:
D. melanogaster (fly)
Entrez Gene:
His4:CG31611 (Dmel_CG31611, CG31611, Dmel\CG31611)
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Vector backbone:
na
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert:
10200
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
EagI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
3' sequencing primer:
CCATCTAATTCAACAAGAATTGGG ACAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Kami Ahmad
Terms and Licenses:
MTA

Comments:

Heat shock promoter. Ahmad lab #k163.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. Ahmad et al (Mol Cell 2002 Jun;9(6):1191-200. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8699" in your Materials and Methods section.