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3120 pSFFV-neo Bcl-XL
(Plasmid #8749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8749 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-xl
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • GenBank ID
    NM_138578
  • Entrez Gene
    BCL2L1 (a.k.a. BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gabriel Nunez
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3120 pSFFV-neo Bcl-XL was a gift from Stanley Korsmeyer (Addgene plasmid # 8749 ; http://n2t.net/addgene:8749 ; RRID:Addgene_8749)
  • For your References section:

    Bcl-XL and Bcl-2 repress a common pathway of cell death. Chao DT, Linette GP, Boise LH, White LS, Thompson CB, Korsmeyer SJ. J Exp Med 1995 Sep 1;182(3):821-8. 10.1084/jem.182.3.821 PubMed 7650488