3789 pmko-neo RNAi-GFP
(Plasmid
#8881)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepmko-neo
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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Alt nameshRNA-GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's sequencing results suggest that the shRNA sequence targeting GFP is CTACGTCCAGGAGCGCACC.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3789 pmko-neo RNAi-GFP was a gift from Stanley Korsmeyer (Addgene plasmid # 8881 ; http://n2t.net/addgene:8881 ; RRID:Addgene_8881) -
For your References section:
Conditional MLL-CBP targets GMP and models therapy-related myeloproliferative disease. Wang J, Iwasaki H, Krivtsov A, Febbo PG, Thorner AR, Ernst P, Anastasiadou E, Kutok JL, Kogan SC, Zinkel SS, Fisher JK, Hess JL, Golub TR, Armstrong SA, Akashi K, Korsmeyer SJ. EMBO J. 2005 Jan 26. 24(2):368-81. 10.1038/sj.emboj.7600521 PubMed 15635450