pTYB11-PTBP1-RRM2CCtoSS
(Plasmid #89154)

• Purpose: recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli
• Depositing Lab: Frédéric Allain
• Publication: Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235.

Backbone

• Vector backbone: pTYB11
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 7412
• Total vector size (bp): 7800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S
• Alt name: PTBP1 RNA Binding Domain 2 mutant C250S, C251S
• Species: H. sapiens (human)
• Insert Size (bp): 420
• Mutation: mutations: Cys 250 to Ser, Cys 251 to Ser
• Entrez Gene: PTBP1 (a.k.a. HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
• Promoter: T7
• Tags / Fusion Proteins:
  • chitin binding domain (N terminal on backbone)
  • Intein (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SapI (unknown if destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christophe Maris

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry