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1072 pBabe puroL myr Akt K179M T308A S473A
(Plasmid #9015)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 9015 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe puro L
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Akt1 K179M T308A S473A
  • Alt name
    Akt1
  • Alt name
    Akt
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    K179M T308A S473A
  • Entrez Gene
    AKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Tags / Fusion Proteins
    • Myr (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBabe5
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1072 pBabe puroL myr Akt K179M T308A S473A was a gift from William Sellers (Addgene plasmid # 9015 ; http://n2t.net/addgene:9015 ; RRID:Addgene_9015)
  • For your References section:

    Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. DiRenzo J, Signoretti S, Nakamura N, Rivera-Gonzalez R, Sellers W, Loda M, Brown M. Cancer Res. 2002 Jan 1. 62(1):89-98. PubMed 11782364