1071 pBabe puroL myr Akt T308A S473A
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||9014||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBabe puro L
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAkt1 T308A S473A
SpeciesH. sapiens (human)
Insert Size (bp)1500
Entrez GeneAKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
/ Fusion Proteins
- Myr (N terminal on backbone)
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBabe5 (Common Sequencing Primers)
The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1071 pBabe puroL myr Akt T308A S473A was a gift from William Sellers (Addgene plasmid # 9014 ; http://n2t.net/addgene:9014 ; RRID:Addgene_9014)
For your References section:Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. DiRenzo J, Signoretti S, Nakamura N, Rivera-Gonzalez R, Sellers W, Loda M, Brown M. Cancer Res. 2002 Jan 1. 62(1):89-98. PubMed 11782364
Map uploaded by the depositor.