pRS313-Sad1-Wt
(Plasmid
#90308)
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Purposeyeast expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS313
- Backbone size w/o insert (bp) 4967
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSAD1
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Alt nameYFR005C
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1346
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Tag
/ Fusion Protein
- no tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SAD1 gene with 800 bp upstream of the start site and 900 bp downstream from the stop codon also included
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS313-Sad1-Wt was a gift from Christine Guthrie (Addgene plasmid # 90308 ; http://n2t.net/addgene:90308 ; RRID:Addgene_90308) -
For your References section:
The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing. Hadjivassiliou H, Rosenberg OS, Guthrie C. RNA. 2014 May;20(5):656-69. doi: 10.1261/rna.042838.113. Epub 2014 Mar 28. 10.1261/rna.042838.113 PubMed 24681967
