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pSupAR-MbPylRS(DiZPK)
(Plasmid #91705)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91705 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pylS
  • Species
    Methanosarcina barkeri
  • Insert Size (bp)
    1260
  • Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    pylT
  • Species
    Methanosarcina barkeri

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

Plasmid for encoding the Methanosarcina barkeri pyrrolysyl-tRNA synthetase (MbPylRS)/tRNA pair in E. coli which can incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into a in-frame amber codon site on a protein of interest.The aminoacyl-tRNA synthetase is engineered to recognize the UAA but not the natural native amino acids, and charge its cognate tRNA with the corresponding UAA. Then, the charged tRNA will insert the UAA at an in-frame amber codon position on a target gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSupAR-MbPylRS(DiZPK) was a gift from Peng Chen (Addgene plasmid # 91705 ; http://n2t.net/addgene:91705 ; RRID:Addgene_91705)
  • For your References section:

    A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance. Zhang M, Lin S, Song X, Liu J, Fu Y, Ge X, Fu X, Chang Z, Chen PR. Nat Chem Biol. 2011 Sep 4;7(10):671-7. doi: 10.1038/nchembio.644. 10.1038/nchembio.644 PubMed 21892184