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pEff
(Plasmid #97423)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 97423 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

Mismatches in GFP are not a concern for plasmid function; intron in GFP prevents recognition by analytical software.

Addgene Note: Large plasmid may be unstable in DH5alpha. We suggest screening colonies before use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEff was a gift from Nikolai Ravin (Addgene plasmid # 97423 ; http://n2t.net/addgene:97423 ; RRID:Addgene_97423)
  • For your References section:

    Efficient Transient Expression of Recombinant Proteins in Plants by the Novel pEff Vector Based on the Genome of Potato Virus X. Mardanova ES, Blokhina EA, Tsybalova LM, Peyret H, Lomonossoff GP, Ravin NV. Front Plant Sci. 2017 Feb 28;8:247. doi: 10.3389/fpls.2017.00247. eCollection 2017. 10.3389/fpls.2017.00247 PubMed 28293244