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C321.ΔClpS.Ub-UAG-sfGFP
(Bacterial strain #98565)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 98565 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    C321.deltaA genome (genomically integrated construct) with ClpS inactivated
  • Backbone size w/o insert (bp) 4600000
  • Modifications to backbone
    A reporter construct was integrated into an intergenic region of the E. coli genome. Subsequently, the N-end rule adaptor protein ClpS was inactivated using MAGE (insertion of multiple premature stop codons).
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    C321.deltaA
  • Growth instructions
    The C321.deltaA strain contains an integrated lambda red cassette under expression by heat shock. Growth at 34 C or lower is recommended to prevent leaky expression of lambda red.
  • Copy number
    Unknown

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.ΔClpS.Ub-UAG-sfGFP was a gift from George Church (Addgene plasmid # 98565)
  • For your References section:

    Engineering posttranslational proofreading to discriminate nonstandard amino acids. Kunjapur AM, Stork DA, Kuru E, Vargas-Rodriguez O, Landon M, Soll D, Church GM. Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):619-624. doi: 10.1073/pnas.1715137115. Epub 2018 Jan 4. 10.1073/pnas.1715137115 PubMed 29301968