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Engineering posttranslational proofreading to discriminate nonstandard amino acids.
Kunjapur AM, Stork DA, Kuru E, Vargas-Rodriguez O, Landon M, Soll D, Church GM
Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):619-624. doi: 10.1073/pnas.1715137115. Epub 2018 Jan 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98564C321.Ub-UAG-sfGFPRecoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter
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98565C321.ΔClpS.Ub-UAG-sfGFPRecoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated
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98566pZE21/UBP1/ClpSExpresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon
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98567pZE21/UBP1/ClpS_V65IExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
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