Addgene: Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum.

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Michalis Averof
Gilles et al

Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum.
Gilles AF, Schinko JB, Averof M
Development. 2015 Jul 9. pii: dev.125054. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
65955	p(U6a-BsaI-gRNA)	Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence
65956	p(U6b-BsaI-gRNA)	Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence
65959	p(bhsp68-Cas9)	Tribolium basal heat shock promoter driving Cas9

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