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(Plasmid #65956)


Item Catalog # Description Quantity Price (USD)
Plasmid 65956 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 2932
  • Vector type
    Insect Expression
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    BsaI cloning site and tracRNA are derived from Addgene DR274.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

An online tool to help oligo design for gRNAs is at

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p(U6b-BsaI-gRNA) was a gift from Michalis Averof (Addgene plasmid # 65956 ; ; RRID:Addgene_65956)
  • For your References section:

    Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum. Gilles AF, Schinko JB, Averof M. Development. 2015 Jul 9. pii: dev.125054. 10.1242/dev.125054 PubMed 26160901