Purpose(Empty Backbone) Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65956||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2932
Vector typeInsect Expression
- Promoter U6
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
An online tool to help oligo design for gRNAs is at http://www.averof-lab.org/tools/Tribolium_CRISPR.php
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p(U6b-BsaI-gRNA) was a gift from Michalis Averof (Addgene plasmid # 65956 ; http://n2t.net/addgene:65956 ; RRID:Addgene_65956)
For your References section:Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum. Gilles AF, Schinko JB, Averof M. Development. 2015 Jul 9. pii: dev.125054. 10.1242/dev.125054 PubMed 26160901