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Optogenetic acidification of synaptic vesicles and lysosomes.
Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
70111pLenti-Synapsin-pHoenix-WPRElentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles
70112pCMV-lyso-pHoenixExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
70113pCMV-lyso-pHluorinExpression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification