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Addgene
A Modular Assembly Platform for Rapid Generation of DNA Constructs.
Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. PubMed Article

Plasmids from Article

ID Plasmid Purpose
68408TOPO 2-5TOPO backbone for GMAP genes with sites #2 and #5
68409TOPO 1-4TOPO backbone for GMAP promoters with sites #1 and #4
68410RV 2-5Retroviral backbone for use with GMAP
68411LV 1-5Lentiviral backbone for use with GMAP
68412R26TV CAG LSL 2-5Rosa26 targeting vector backbone for use with GMAP
68413TOPO mTagBFP2TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene
68441TOPO mKate2TOPO construct expressing mKate2 for generation of GMAP-compatible gene
68442TOPO mKO2TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene
68443TOPO iRFP670TOPO construct expressing iRFP670 for generation of GMAP-compatible gene
68444TOPO CloverCPTOPO construct expressing CloverCP for generation of GMAP-compatible gene
68445TOPO HygroRTOPO construct expressing HygroR for generation of GMAP-compatible gene
68446TOPO ZeoRTOPO construct expressing ZeoR for generation of GMAP-compatible gene
68447TOPO PuroRTOPO construct expressing PuroR for generation of GMAP-compatible gene
68448TOPO rtTA3-2A-CreTOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene
68449TOPO tTR-KRAB-rtTA3-LucTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene
68450TOPO Cre-2A-GFPTOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene
68451TOPO EFSTOPO construct expressing EFS for generation of GMAP-compatible promoter
68452TOPO pGKTOPO construct expressing pGK for generation of GMAP-compatible promoter
68453TOPO CCSPTOPO construct expressing CCSP for generation of GMAP-compatible promoter
68454TOPO CMVTOPO construct expressing CMV for generation of GMAP-compatible promoter
68455TOPO SV40TOPO construct expressing SV40 for generation of GMAP-compatible promoter
68456TOPO UBCTOPO construct expressing UBC for generation of GMAP-compatible promoter
68457TOPO pTRETOPO construct expressing pTRE for generation of GMAP-compatible promoter
68458TOPO HRETOPO construct expressing HRE for generation of GMAP-compatible promoter
68464FFiBlast MSCV PuroRetroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line
68469MSCV PuroRetroviral plasmid with multiple cloning site and puromycin resistance
68470FFiBlast p5Inverted and floxed blasticidin resistance
68471pTRE:shRNA A-pGK:rtTA-CreInducible lentivirus expressing an arbitrary shRNA and Cre
68475pGK:TRL-EFS:iRFP670Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670
68476R26TV LSL-TRLRosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette
68477pTRE:iRFP670-EFS:Cre-2A-GFPInducible lentivirus expressing iRFP670, Cre, and GFP
68478pTRE:CloverCP-EFS:ZeoRLentivirus for establishment of doxycycline-inducible cell line expression CloverCP
68479pGK:HygroR-CMV:mTagBFP2-A_UTRLentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance
68480pGK:HygroR-CMV:mKate2-B_UTRLentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance
68481pGK:HygroR-CMV:mKO2-C_UTRLentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance
68482MSCV Puro-UBC:GFPRetrovirus expressing GFP driven by UBC promoter
68483MSCV Puro-SV40:GFPRetrovirus expressing GFP driven by SV40 promoter
68484MSCV Puro-EFS:GFPRetrovirus expressing GFP driven by EFS promoter
68485MSCV Puro-CMV:GFPRetrovirus expressing GFP driven by CMV promoter
68486MSCV Puro-pGK:GFPRetrovirus expressing GFP driven by pGK promoter
68487MSCV Puro-CCSP:GFPRetrovirus expressing GFP driven by CCSP promoter
68488TOPO GFPTOPO construct expressing GFP for generation of GMAP-compatible gene
68489TOPO CFPTOPO construct expressing CFP for generation of GMAP-compatible gene
68490TOPO mCherryTOPO construct expressing mCherry for generation of GMAP-compatible gene
68491TOPO mT2TOPO construct expressing mT2 for generation of GMAP-compatible gene
68493TOPO VenusTOPO construct expressing Venus for generation of GMAP-compatible gene

Antibodies from Article