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A Modular Assembly Platform for Rapid Generation of DNA Constructs.
Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68408TOPO 2-5TOPO backbone for GMAP genes with sites #2 and #5
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68409TOPO 1-4TOPO backbone for GMAP promoters with sites #1 and #4
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68410RV 2-5Retroviral backbone for use with GMAP
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68411LV 1-5Lentiviral backbone for use with GMAP
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68412R26TV CAG LSL 2-5Rosa26 targeting vector backbone for use with GMAP
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68413TOPO mTagBFP2TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene
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68441TOPO mKate2TOPO construct expressing mKate2 for generation of GMAP-compatible gene
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68442TOPO mKO2TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene
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68443TOPO iRFP670TOPO construct expressing iRFP670 for generation of GMAP-compatible gene
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68444TOPO CloverCPTOPO construct expressing CloverCP for generation of GMAP-compatible gene
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68445TOPO HygroRTOPO construct expressing HygroR for generation of GMAP-compatible gene
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68446TOPO ZeoRTOPO construct expressing ZeoR for generation of GMAP-compatible gene
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68447TOPO PuroRTOPO construct expressing PuroR for generation of GMAP-compatible gene
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68448TOPO rtTA3-2A-CreTOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene
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68449TOPO tTR-KRAB-rtTA3-LucTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene
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68450TOPO Cre-2A-GFPTOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene
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68451TOPO EFSTOPO construct expressing EFS for generation of GMAP-compatible promoter
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68452TOPO pGKTOPO construct expressing pGK for generation of GMAP-compatible promoter
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68453TOPO CCSPTOPO construct expressing CCSP for generation of GMAP-compatible promoter
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68454TOPO CMVTOPO construct expressing CMV for generation of GMAP-compatible promoter
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68455TOPO SV40TOPO construct expressing SV40 for generation of GMAP-compatible promoter
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68456TOPO UBCTOPO construct expressing UBC for generation of GMAP-compatible promoter
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68457TOPO pTRETOPO construct expressing pTRE for generation of GMAP-compatible promoter
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68458TOPO HRETOPO construct expressing HRE for generation of GMAP-compatible promoter
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68464FFiBlast MSCV PuroRetroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line
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68469MSCV PuroRetroviral plasmid with multiple cloning site and puromycin resistance
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68470FFiBlast p5Inverted and floxed blasticidin resistance
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68471pTRE:shRNA A-pGK:rtTA-CreInducible lentivirus expressing an arbitrary shRNA and Cre
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68473pTRE:shRNA B-pGK:rtTA-CreInducible lentivirus expressing an arbitrary shRNA and Cre
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68475pGK:TRL-EFS:iRFP670Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670
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68476R26TV LSL-TRLRosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette
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68477pTRE:iRFP670-EFS:Cre-2A-GFPInducible lentivirus expressing iRFP670, Cre, and GFP
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68478pTRE:CloverCP-EFS:ZeoRLentivirus for establishment of doxycycline-inducible cell line expression CloverCP
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68479pGK:HygroR-CMV:mTagBFP2-A_UTRLentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance
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68480pGK:HygroR-CMV:mKate2-B_UTRLentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance
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68481pGK:HygroR-CMV:mKO2-C_UTRLentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance
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68482MSCV Puro-UBC:GFPRetrovirus expressing GFP driven by UBC promoter
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68483MSCV Puro-SV40:GFPRetrovirus expressing GFP driven by SV40 promoter
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68484MSCV Puro-EFS:GFPRetrovirus expressing GFP driven by EFS promoter
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68485MSCV Puro-CMV:GFPRetrovirus expressing GFP driven by CMV promoter
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68486MSCV Puro-pGK:GFPRetrovirus expressing GFP driven by pGK promoter
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68487MSCV Puro-CCSP:GFPRetrovirus expressing GFP driven by CCSP promoter
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68488TOPO GFPTOPO construct expressing GFP for generation of GMAP-compatible gene
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68489TOPO CFPTOPO construct expressing CFP for generation of GMAP-compatible gene
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68490TOPO mCherryTOPO construct expressing mCherry for generation of GMAP-compatible gene
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68491TOPO mT2TOPO construct expressing mT2 for generation of GMAP-compatible gene
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68493TOPO VenusTOPO construct expressing Venus for generation of GMAP-compatible gene
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