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N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.
Sekar K, Gentile AM, Bostick JW, Tyo KE
PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
PubMed

Plasmids from Article

ID Plasmid Purpose  
65464pKS011pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
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65465pKS012pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry
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65466pKS013pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry
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65467pKS014pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase
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65468pJB005pTrc plasmid expressing GFP
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65469pJB028pTrc plasmid expressing p14*-TEV
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65470pKS042pTrc plasmid expressing clpA
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65471pKS043pTrc plasmid expressing clpS
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65472pKS044pTrc plasmid expressing clpP
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65915MG1655 Z1 malEE. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO
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65917MG1655 Z1 malE aatE. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO
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65918MG1655 Z1 malE clpAE. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO
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