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Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR-Cas9 genome-editing efficiency.
Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 2017 Nov 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74939pcDNA3-Flag::UbvG08 I44A, deltaGGUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
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74940pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGGUbiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)
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75030pMX-HA-UbvG08-IRES-GFPRetroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)
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75031pMX-HA-UbvG08_MUT-IRES-GFPRetroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)
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92170pAAV-i53recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1
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92171pAAV-i53-DMrecombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
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