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An Optical Neuron-Astrocyte Proximity Assay at Synaptic Distance Scales.
Octeau JC, Chai H, Jiang R, Bonanno SL, Martin KC, Khakh BS
Neuron. 2018 Apr 4;98(1):49-66.e9. doi: 10.1016/j.neuron.2018.03.003.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92277pcDNA3.1 CMV NAPA-A BGHFRET probe; extracellular membrane tethered eGFP
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92278pcDNA 3.1 CMV NAPA-N BGHFRET probe; extracellular synapse targeted mCherry
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92280pcDNA3.1 CMV mCherry-eGFP BGHControl FRET construct; tandem mCherry-eGFP fusion
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92281pZac2.1 GfaABC1D NAPA-A SV40FRET probe; extracellular membrane tethered eGFP
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92282pZac2.1 hSynapsin1 NAPA-N SV40FRET probe; extracellular synapse targeted mCherry
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92283pZac2.1 hSynapsin1 FLEX NAPA-N SV40FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed
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97212pZac2.1 hSynapsin1 NAPA-N2 SV40FRET probe; extracelllular synapse targeted mScarlet
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Antibodies from Article