pZac2.1 hSynapsin1 NAPA-N2 SV40
PurposeFRET probe; extracelllular synapse targeted mScarlet
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||97212||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameextracellular membrane tethered fluorescent protein
- Promoter hSynapsin1
- Cloning method Gibson Cloning
- 5′ sequencing primer CTT GTA CAG CTC GTC CAT GCC CAT ACT CAA GCT TCT TGT AC
- 3′ sequencing primer GTG GTT TGT CCA AAC TCA TCA AT (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZac2.1 hSynapsin1 NAPA-N2 SV40 was a gift from Baljit Khakh (Addgene plasmid # 97212 ; http://n2t.net/addgene:97212 ; RRID:Addgene_97212)
For your References section:An Optical Neuron-Astrocyte Proximity Assay at Synaptic Distance Scales. J. Christopher Octeau, Hua Chai, Ruotian Jiang, Shivan L. Bonanno, Kelsey C. Martin, Baljit S. Khakh. Neuron (Volume 98, Issue 1, p49–66) 10.1016/j.neuron.2018.03.003