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Local control of intracellular microtubule dynamics by EB1 photodissociation.
van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann T
Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 10.1038/s41556-017-0028-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
107614pEB1N-LZ-LOV2(wt)N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag
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107690pEB1N-LZ-LOV2(fast)N-terminal half of π-EB1 with fast LOV2, no fluorescent tag
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107693pEB1N-LZ-mCherry-LOV2(wt)N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends
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107695pmCherry-Zdk1-EB1CC-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light
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107696pEGFP-Zdk1-EB1CC-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light
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107726pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1Knock out of EB1 in human cells by CRISPR/Cas9
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107727pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2Knock out of EB1 in human cells by CRISPR/Cas9
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107728pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3Knock out of EB1 in human cells by CRISPR/Cas9
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107729pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1 Knock out of EB3 in human cells by CRISPR/Cas9
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107730pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2Knock out of EB3 in human cells by CRISPR/Cas9
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107731pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3Knock out of EB3 in human cells by CRISPR/Cas9
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