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Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.
Casler JC, Papanikou E, Barrero JJ, Glick BS
J Cell Biol. 2019 May 6;218(5):1582-1601. doi: 10.1083/jcb.201807195. Epub 2019 Mar 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
115420YIplac204-pOst1-ESCargoExpresses a regulatable secretory cargo for yeast at the TRP1 locus
115421YIplac128-pOst1-ESCargoExpresses a regulatable secretory cargo for yeast at the LEU2 locus
115422YIplac211-Erg11-iGFPx3Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis
115423YIplac211-Sec31-yoHaloExpress Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis
115424pSec7-iGFPx6Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis
115425pSec7-Halox2Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis
115426YIplac211-vps10-104Generate the vps10-104 allele via pop-in pop-out mutagenesis
115427YIplac204-pOst1-ESCargo*Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus
115428YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus
160904YIplac211-sGFP-Vrg4Expresses GFP-Vrg4 in yeast cells

Antibodies from Article