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Efficient targeted integration directed by short homology in zebrafish and mammalian cells.
Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner J
Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968. PubMed Article

Plasmids from Article

ID Plasmid Purpose
117765pPRISM-TagRFP-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117766pPRISM-TagRFP-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117767pPRISM-TagRFP-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117768pPRISM-TagRFP-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117769pPRISM-TagRFP-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117770pPRISM-TagRFP-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117771pPRISM-Stop-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117772pPRISM-Stop-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117773pPRISM-Stop-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117774pPRISM-Stop-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117775pPRISM-Stop-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117776pPRISM-Stop-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117777pPRISM-gal4-VP16-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117778pPRISM-gal4-VP16-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117779pPRISM-gal4-VP16-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117780pPRISM-gal4-VP16-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117781pPRISM-gal4-VP16-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117782pPRISM-gal4-VP16-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117783pPRISM-Fuse-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117784pPRISM-Fuse-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117785pPRISM-Fuse-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117786pPRISM-Fuse-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117787pPRISM-Fuse-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117788pPRISM-Fuse-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117789pPRISM-Cre-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117790pPRISM-Cre-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117791pPRISM-Cre-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117792pPRISM-Cre-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117793pPRISM-Cre-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117794pPRISM-Cre-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
117801Control-cx43.4-48H-pPRISMControl to test technique on a target that is known to work
117805pGTag-NLS-TagRFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
117806pGTag-NLS-TagRFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117807pGTag-TagRFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
117808pGTag-TagRFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117809pGTag-TagRFP-CAAX-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
117810pGTag-TagRFP-CAAX-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117811pGTag-NLS-eGFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
117812pGTag-NLS-eGFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117813pGTag-eGFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
117814pGTag-eGFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117815pGTag-eGFP-CAAX-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
117816pGTag-eGFP-CAAX-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117817pGTag-gal4-VP16-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
117818Control-cx43.4-48H-pGTagDonor for precise CRISPR directed genomic integration using the GeneWeld method

Antibodies from Article