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Efficient targeted integration directed by short homology in zebrafish and mammalian cells.
Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner J
Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117765pPRISM-TagRFP-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117766pPRISM-TagRFP-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117767pPRISM-TagRFP-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117768pPRISM-TagRFP-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117769pPRISM-TagRFP-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117770pPRISM-TagRFP-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117771pPRISM-Stop-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117772pPRISM-Stop-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117773pPRISM-Stop-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117774pPRISM-Stop-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117775pPRISM-Stop-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117776pPRISM-Stop-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117777pPRISM-gal4-VP16-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117778pPRISM-gal4-VP16-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117779pPRISM-gal4-VP16-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117780pPRISM-gal4-VP16-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117781pPRISM-gal4-VP16-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117782pPRISM-gal4-VP16-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117783pPRISM-Fuse-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117784pPRISM-Fuse-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117785pPRISM-Fuse-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117786pPRISM-Fuse-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117787pPRISM-Fuse-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117788pPRISM-Fuse-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117789pPRISM-Cre-gcry-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117790pPRISM-Cre-gcry-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117791pPRISM-Cre-gcry-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117792pPRISM-Cre-cmlc2-TagBFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117793pPRISM-Cre-cmlc2-mRFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117794pPRISM-Cre-cmlc2-eGFPDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117801Control-cx43.4-48H-pPRISMControl to test technique on a target that is known to work
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117805pGTag-NLS-TagRFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
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117806pGTag-NLS-TagRFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117807pGTag-TagRFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
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117808pGTag-TagRFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117810pGTag-TagRFP-CAAX-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117811pGTag-NLS-eGFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
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117812pGTag-NLS-eGFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117813pGTag-eGFP-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
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117814pGTag-eGFP-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117815pGTag-eGFP-CAAX-SV40Donor for precise CRISPR directed genomic integration using the GeneWeld method
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117816pGTag-eGFP-CAAX-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117817pGTag-gal4-VP16-B-actinDonor for precise CRISPR directed genomic integration using the GeneWeld method
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117818Control-cx43.4-48H-pGTagDonor for precise CRISPR directed genomic integration using the GeneWeld method
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Recombinant Antibodies from Article