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An intrinsic S/G2 checkpoint enforced by ATR.
Saldivar JC, Hamperl S, Bocek MJ, Chung M, Bass TE, Cisneros-Soberanis F, Samejima K, Xie L, Paulson JR, Earnshaw WC, Cortez D, Meyer T, Cimprich KA
Science. 2018 Aug 24;361(6404):806-810. doi: 10.1126/science.aap9346. PubMed Article

Plasmids from Article

ID Plasmid Purpose
118595CDK1as_T2A_puromycinOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.
118596CDK1as_T2A_ZeoOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
118597pX330_human CDK1One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Antibodies from Article