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Genetically encoded split-luciferase biosensors to measure endosome disruption rapidly in live cells.
Kilchrist KV, Tierney JW, Duvall CL
ACS Sens. 2020 Jun 23. doi: 10.1021/acssensors.0c00103. PubMed Article

Plasmids from Article

ID Plasmid Purpose
128386pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
128387pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption

Antibodies from Article