Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.
Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD
Viruses. 2020 May 6;12(5). pii: v12050513. doi: 10.3390/v12050513. PubMed Article
Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD
Viruses. 2020 May 6;12(5). pii: v12050513. doi: 10.3390/v12050513. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155130 | HDM-SARS2-Spike-delta21 | Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping |
| 158762 | HDM_SARS2_Spike_del21_D614G | Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614 |