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Mapping the dynamic transfer functions of eukaryotic gene regulation.
Lee JB, Caywood LM, Lo JY, Levering N, Keung AJ
Cell Syst. 2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 10.1016/j.cels.2021.08.003. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174063pJL29Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.
174064pJL30Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
174066pJL32Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.
174067pJL45Expresses CIB1 in S. cerevisiae. Includes inducible lac operon.
174068pJL50Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.
174069pJL15Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
174070pJL1Expresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.

Antibodies from Article