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The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase.
van Beljouw SPB, Haagsma AC, Rodriguez-Molina A, van den Berg DF, Vink JNA, Brouns SJJ
Science. 2021 Sep 17;373(6561):1349-1353. doi: 10.1126/science.abk2718. Epub 2021 Aug 26. PubMed Article

Plasmids from Article

ID Plasmid Purpose
178980pTU39813S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)
178981pTU387pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array
178982pTU42213S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)
178983pTU424pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)
178984pTU425pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

Antibodies from Article