The viral packaging motor potentiates Kaposi's sarcoma-associated herpesvirus gene expression late in infection.
McCollum CO, Didychuk AL, Liu D, Murray-Nerger LA, Cristea IM, Glaunsinger BA
PLoS Pathog. 2023 Apr 17;19(4):e1011163. doi: 10.1371/journal.ppat.1011163. eCollection 2023 Apr. PubMed Article
McCollum CO, Didychuk AL, Liu D, Murray-Nerger LA, Cristea IM, Glaunsinger BA
PLoS Pathog. 2023 Apr 17;19(4):e1011163. doi: 10.1371/journal.ppat.1011163. eCollection 2023 Apr. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200017 | pCDNA4.TO_ORF18_TurboID_3xHA | Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA |
| 200018 | pCDNA4.TO_ORF30_TurboID_3xHA | Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA |
| 200019 | pCDNA4.TO_ORF31_TurboID_3xHA | Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA |
| 200020 | pCDNA4.TO_ORF66_TurboID_3xHA | Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA |
| 200021 | pCDNA4.TO_3xHA_TurboID_ORF34 | Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID |
| 200022 | pLVX-TetOne-zeo_3xHA-TurboID-NLS | For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter |
| 200024 | pLJM1-zeo G226A ORF29 | Lentiviral vector for expression of KSHV ORF29 with G226A mutation |
| 200025 | pLJM1-zeo E321K ORF29 | Lentiviral vector for expression of KSHV ORF29 with E321K mutation |
| 200026 | pLJM1-zeo G226A E321K ORF29 | Lentiviral vector for expression of KSHV ORF29 with G226A and E321K mutation |
| 200027 | pLJM1-zeo D476A ORF29 | Lentiviral vector for expression of KSHV ORF29 with D476A mutation |
| 200028 | pLJM1-zeo-ORF29 | Lentiviral vector for expression of KSHV ORF29 |
| 200060 | pDM002-sgRNA-lenti-ZeoR | Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites |