A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis.
Capece M, Tessari A, Mills J, Vinciguerra GLR, Louke D, Lin C, McElwain BK, Miles WO, Coppola V, Davies AE, Palmieri D, Croce CM
Cell Death Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4. PubMed Article
Capece M, Tessari A, Mills J, Vinciguerra GLR, Louke D, Lin C, McElwain BK, Miles WO, Coppola V, Davies AE, Palmieri D, Croce CM
Cell Death Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171092 | pDP1.2 | Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor) |
| 171094 | pLentiDP1.0 | lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2 |
| 171095 | pLentiDP1.2 | lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2 |
| 171096 | pLentiDP1.3 | lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2 |
| 171097 | pDP20 | Expresses mAID-tagged mCherry2 |