A genetically encoded photoactivatable Rac controls the motility of living cells.
Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM
Nature. 2009 Sep 3. 461(7260):104-8. PubMed Article
Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM
Nature. 2009 Sep 3. 461(7260):104-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 22007 | pTriEx-mVenus-PA-Rac1 | Expression of photoactivatable Rac1 |
| 22008 | pBabe-TetCMV-puro-mVenus-PA-Rac1 | Mammalian expression of photoactivatable Rac1 |
| 22017 | pTriEx-mVenus-PA-Rac1-T17N | Expression of photoactivatable Rac1 (dominant-negative mutant) |
| 22018 | pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N | |
| 22021 | pTriEx-mVenus-PA-Rac1-C450A | Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation) |
| 22022 | pTriEx-mPA-GFP-PA-Rac1 | Expression of photoactivatable Rac1 |
| 22023 | pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A | |
| 22024 | pTriEx-PA-Rac1 | Expression of photoactivatable Rac1 |
| 22025 | pTriEx-PA-Rac1-C450A | Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation) |
| 22026 | pTriEx-PA-Rac1-I539E | Expression of photoactivatable Rac1 ('lit' state mutant) |
| 22027 | pTriEx-mCherry-PA-Rac1 | Expression of photoactivatable Rac1 |
| 22028 | pTriEx-mCherry-PA-Rac1-C450A | Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation) |
| 22029 | pTriEx-mCherry-PA-Rac1-T17N | Expression of photoactivatable Rac1 (dominant-negative mutant). |
| 22030 | pTriEx-mCerulean-PA-Rac1 | Expression of photoactivatable Rac1 |
| 22031 | pBabe-TetCMV-puro-mCerulean-PA-Rac1 | |
| 22032 | pBabe-TetCMV-puro-mPA-GFP-PA-Rac1 | |
| 22033 | pBabe-TetCMV-puro-mVenus-LOV | Mammalian expression of mVenus tagged LOV2 domain |
| 22034 | pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M | |
| 22035 | pBabe-TetCMV-puro-mCherry-PA-Rac1 | Expression of photoactivatable Rac1 |
| 75263 | PA-Cdc42 | Light-activated Cdc42 |