This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
PubMed Article

By studying a collection of 582 natural and synthetic terminators, Christopher Voigt’s lab has characterized the strength of various terminators and identified design constraints required to build strong, reliable synthetic terminators. This expands the library of terminators available for use in synthetic biology circuits.

This publication describes the measurements made on 227 E. coli terminators, 90 reverse terminators, and 265 synthetic terminators. The terminator strength was measured by developing assay reporter plasmids (shown below), where the terminator of interest was inserted between the GFP and RFP, and comparing the induced GFP and induced RFP output. Using these measurements, Chen et al. modeled the contribution of the various features (U-tract, A-tract, hairpin, etc.) on terminator strength and designed new synthetic terminators.

Voigt Terminator Project Graphic

The Voigt lab has deposited 10 of the described plasmids to Addgene. These include the empty backbone, pGR, and 9 variations of pGR-** with various terminators inserted between the fluorescent proteins (where ** is the specific terminator). The sequences and corresponding data for all 582 terminators can be found below.

Resources

Downloadable data and sequences for all 582 terminators:

Plasmids from Article

ID Plasmid  
46002pGR
Loading...
46003pGR-BBa_B0010
Loading...
46004pGR-L3S2P21
Loading...
46005pGR-L3S2P00
Loading...
46006pGR-ECK120015170
Loading...
46007pGR-L3S2P36
Loading...
46008pGR-ECK125108723
Loading...
46009pGR-ECK120010783
Loading...
46010pGR-ECK120010800
Loading...
46011pGR-ECK120033127
Loading...