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Targeted genome modifications in soybean with CRISPR/Cas9.
Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47024pUC gRNA ShuttleEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
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59175p201N Cas9Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
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59176p201H Cas9Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
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59177p201B Cas9Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
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59178p201G Cas9Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
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Antibodies from Article