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Genetic screens in human cells using the CRISPR-Cas9 system.
Wang T, Wei JJ, Sabatini DM, Lander ES
Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50661pCW-Cas9Doxycycline-inducible lentiviral expression of SpCas9
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50662pLX-sgRNALentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)
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51043Human Lentiviral sgRNA Library - Unknown FunctionSub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function.
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51044Human Lentiviral sgRNA Library - KinasesSub-pool of Sabatini/Lander sgRNA library enriched for kinase targets.
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51045Human Lentiviral sgRNA Library - Ribosomal ProteinsSub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets.
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51046Human Lentiviral sgRNA Library - Cell CycleSub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets.
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51047Human Lentiviral sgRNA Library - NuclearSub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.
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51048Human Lentiviral sgRNA Library - ControlSub-pool of Sabatini/Lander sgRNA library enriched for control targets.
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Antibodies from Article